|
KMID : 0545120080180050885
|
|
Journal of Microbiology and Biotechnology
2008 Volume.18 No. 5 p.885 ~ p.888
|
|
|
Continuous Cell-Free Protein Synthesis Using Glycolytic Intermediates as Energy Sources
|
|
Kim Ho-Cheol
Kim Tae-Wan
Park Chang-Gil
Oh In-Seok
Park Kyung-Moon
Kim Dong-Myung
|
|
|
Abstract
|
|
|
|
In this work, we demonstrate that glycolytic intermediates can serve as efficient energy sources to regenerate ATP during continuous-exchange cell-free (CECF) protein synthesis reactions. Through the use of an optimal energy source, approximately 10 mg/ml of protein was generated from CECF protein synthesis reaction at greatly reduced reagent costs. Compared with the conventional reactions utilizing phosphoenol pyruvate as an energy source, the described method yields 10-fold higher productivity per unit reagent cost, making the techniques of CECF protein synthesis more realistic alternative for rapid protein production.
|
|
|
KEYWORD
|
|
|
Glycolysis, ATP regeneration, S12 extract, cellfree protein synthesis, fructose-1, 6-bisphosphate
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|